Dielectrophoresis (DEP) of Cells and Microparticle in PDMS Microfluidic Channels

نویسندگان

  • Thirukumaran T. Kanagasabapathi
  • Christopher J. Backhouse
  • Karan V.I.S. Kaler
چکیده

Electromanipulation of microparticles utilizing microelectrodes has demonstrated considerable promise for the characterization, separation and handling of biological cells. Presently considerable research and development effort is directed towards the development of miniaturized fluidic systems with integrated dielectrophoresis (DEP) electrodes. The design, development and fabrication of a DEP microfluidic assembly with an in-built interdigitated microelectrode array is presented. Continuous fractionation of microparticles in a PDMS microfluidic channel is described. Experimental verification of positive and negative DEP of yeast cells and polystyrene latex beads is demonstrated. 1 Corresponding author.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Microfluidic characterization and continuous separation of cells and particles using conducting poly(dimethyl siloxane) electrode induced alternating current-dielectrophoresis.

This paper presents a poly(dimethyl siloxane) (PDMS) polymer microfluidic device using alternating current (ac) dielectrophoresis (DEP) for separating live cells from interfering particles of similar sizes by their polarizabilities under continuous flow and for characterizing DEP behaviors of cells in stagnant flow. The ac-DEP force is generated by three-dimensional (3D) conducting PDMS composi...

متن کامل

Rapid Concentration of Nanoparticles with DC Dielectrophoresis in Focused Electric Fields

We report a microfluidic device for rapid and efficient concentration of micro/nanoparticles with direct current dielectrophoresis (DC DEP). The concentrator is composed of a series of microchannels constructed with PDMS-insulating microstructures for efficiently focusing the electric field in the flow direction to provide high field strength and gradient. The location of the trapped and concen...

متن کامل

Off-chip passivated-electrode, insulator-based dielectrophoresis (OπDEP)

In this study, we report the first off-chip passivated-electrode, insulator-based dielectrophoresis microchip (OπDEP). This technique combines the sensitivity of electrode-based dielectrophoresis (eDEP) with the high-throughput and inexpensive device characteristics of insulator-based dielectrophoresis (iDEP). The device is composed of a permanent, reusable set of electrodes and a disposable, p...

متن کامل

Microfluidic mixing using contactless dielectrophoresis.

The first experimental evidence of mixing enhancement in a microfluidic system using contactless dielectrophoresis (cDEP) is presented in this work. Pressure-driven flow of deionized water containing 0.5 μm beads was mixed in various chamber geometries by imposing a dielectrophoresis (DEP) force on the beads. In cDEP the electrodes are not in direct contact with the fluid sample but are instead...

متن کامل

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting and counting cells. However, manipulating the cell within the fluidic domain remains a challenge and requires complicated fabrication protocols for forming valves and electrodes, or demands specialty equipment like optical tweezers. Here, we dem...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2004